Evaluation of Lysinibacillus SP, isolated from Coptotermes curvignathus Gut, for the delignification of oil palm residues

  • Fadilat Ayeronfe Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Johor, Malaysia.
  • Angzzas Kassim Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Johor, Malaysia.
  • Patricia Hung Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia, 97008, Bintulu, Sarawak, Malaysia
  • Hafiz Zainulabidin
  • Nadiah Ishak Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Johor, Malaysia.
  • Sharfina Syarifah Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Johor, Malaysia.
  • Ashuvila Aripin Faculty of Engineering Technology, University Tun Hussein Onn Malaysia, Johor, Malaysia.
Keywords: Biodelignification, Oil palm, Pulping, Lignin, Enzymes

Abstract

The application of ligninolytic bacteria and enzymes is a green pre-treatment alternative in the production of paper and biofuel from oil palm residues. In this study we investigated the ability of Lysinibacillus pakistanensis isolated from termite gut in degrading the lignin component of oil palm residues. The residues were biotreated with the bacterial strain in an aerated submerged fermentation system for 7 days at 30 , pH 7 and compared with untreated control. Enzyme activities were determined using specific substrates. Peak lignin peroxidase (377.6 U/L), manganese peroxidase (218.19 U/L), and laccase (405.4 U/L) activity were recorded after 4,4, and 5 days of incubation respectively, using oil palm leaf as substrates. Lignin loss of 4.5%, 5.7% and 6.6% in oil palm leaf, oil palm trunk and empty fruit bunch respectively was achieved after treatment with the microorganism. SEM images revealed structural changes in the cell wall of the residues. Pre-treatment with this bacterial strain has promising prospects of improving the efficiency of the pulping process in an environmentally safe manner.

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Published
2019-03-25
Section
Agricultural Sciences