Esterase activity in homogenates of Helicoverpa armigera (Hubner) (Lepidoptera: noctuidae) exposed to different insecticides and the behavioral effect

  • Eliane Carneiro Federal University of Piaui http://orcid.org/0000-0003-2618-6977
  • Luciana Barboza Silva Federal University of Piaui
  • Patrícia Paiva Federal University of Recife
  • Thiago Henrique Napoleão Federal University of Recife
  • Gabriel dos santos Carvalho Federal University of Piaui
  • Gleidyane Novais Lopes Federal University of Piaui
  • Bruno Ettore Pavan Faculty of Engineering, State University of São Paulo
Keywords: Enzymatic activity, Detoxification, Resistance, Sublethal effects

Abstract

The toxicity of some insecticides to Helicoverpa armigera was studied through sublethal effects, evaluating the enzymatic activity of esterase and the behavioral response. The commercial formulation of insecticides selected were chlorpyrifos, spinosad, indoxacarb, chlorantraniliprole, lambda-cyhalothrin and Bacillus thuringiensis, corresponding the most used by farmers to control of H. armigera. To determine the esterase activity, the larvae were fed with soybean leaf discs dipped in insecticide solution using the lethal concentration (LC50). For the behavioral response, filter paper were immersed in three concentrations of insecticides (LC50, LC95 and recommend dose.), then the behavior of the larvae observed in Videomex-One. The results for the enzymatic activity showed an increase in the activity of the esterase, with variation along the treatments and the time of exposure of the insects to the insecticides. With exception of spinosad, other insecticides showed an increase in EST-α activity, 6 and 24 hours after contact of caterpillars with insecticide.Different behavioral patterns of walking (walking distance, walking speed and resting time) were observed for H. armigera exposed to different insecticides. H. armigera exposed to chlorpyrifos, lambda-cyhalothrin and B. thuringiensis insecticides show greater esterase activity. Regarding walking behavior, the results confirms enzymatic activity, where H. armigera have behavioral alteration after exposure to insecticide.

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Author Biographies

Eliane Carneiro, Federal University of Piaui
Graduate Program in Agronomy - Planting Science, Federal University of Piaui - Campus Professora Cinobelina Elvas, Bom Jesus - Viana, Km 03, Bom Jesus, PI, 64900-000, Brazil.
Luciana Barboza Silva, Federal University of Piaui
Graduate Program in Agronomy - Planting Science, Federal University of Piaui - Campus Professora Cinobelina Elvas, Bom Jesus - Viana, Km 03, Bom Jesus, PI, 64900-000, Brazil.
Patrícia Paiva, Federal University of Recife
Department of Biochemistry, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235 - Cidade Universitaria, Recife - PE, 50670-901, Brazil
Thiago Henrique Napoleão, Federal University of Recife
Department of Biochemistry, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235 - Cidade Universitaria, Recife - PE, 50670-901, Brazil
Gabriel dos santos Carvalho, Federal University of Piaui
Graduate Program in Agronomy - Planting Science, Federal University of Piaui - Campus Professora Cinobelina Elvas, Bom Jesus - Viana, Km 03, Bom Jesus, PI, 64900-000, Brazil.
Gleidyane Novais Lopes, Federal University of Piaui
Graduate Program in Agronomy - Planting Science, Federal University of Piaui - Campus Professora Cinobelina Elvas, Bom Jesus - Viana, Km 03, Bom Jesus, PI, 64900-000, Brazil.
Bruno Ettore Pavan, Faculty of Engineering, State University of São Paulo
Department of Plant Science Socio-Economics and Food Technology of Ilha Solteira, UNESP-FEIS, Faculty of Engineering, State University of São Paulo, Box 31, Ilha Solteira, SP, 15385-000, Brazil
Published
2019-02-17
How to Cite
Carneiro, E., Silva, L. B., Paiva, P., Napoleão, T. H., Carvalho, G. dos santos, Lopes, G. N., & Pavan, B. E. (2019). Esterase activity in homogenates of Helicoverpa armigera (Hubner) (Lepidoptera: noctuidae) exposed to different insecticides and the behavioral effect. Bioscience Journal, 35(1). https://doi.org/10.14393/BJ-v35n1a2019-41477
Section
Agricultural Sciences