Direct in vitro regeneration of castor bean plants (Ricinus communis) using epicotyls

Keywords: Organogenesis, Acclimatization, Rooting, Tropical Crop, Plant tissue culture, Oilseed crop.

Abstract

A regeneration protocol for castor bean plant (Ricinus communis) was successfully developed using epicotyl sections obtained from in vitro seedlings. The maximum number of induced shoots (4.3 shoots/explant) and highest shoots frequency (75,56%) was obtained in WPM medium supplemented with TDZ (1 mg/L) and zeatin (0.5 mg/L), whereas the minimum number (0.8 shoots/explant) and lowest shoots frequency (37,78%) was obtained in medium containing TDZ (1 mg/L) and BAP (0.5 mg/L). The highest percentage of rooting (93.3%) was obtained in a medium containing IBA (1 mg/L). These plants were transplanted in a mesh house and achieved a high adaptability to acclimatization, reaching 77% survival. On the other hand, the maximum elongation (height) during this stage was 7.9 cm in plants supplemented with WPM nutrients, whereas it was only 4.38 cm in control plants.

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Published
2018-12-18
How to Cite
Gil Correal, A., Restrepo-Osorio, C., Álvarez, J. C., & Villanueva-Mejía, D. F. (2018). Direct in vitro regeneration of castor bean plants (Ricinus communis) using epicotyls. Bioscience Journal, 35(2). https://doi.org/10.14393/BJ-v35n2a20198-41475
Section
Agricultural Sciences