Concentration of ms medium and cutting of seeds on in vitro establishment of baruzeiro (Dipteryx alata Vog.)

  • Hernane Fernandes Pinhal Universidade Federal de Uberlândia
  • Elequisandra da Costa Araruna Universidade Federal de Uberlândia
  • Pedro Augusto Porto Carneiro Universidade Federal de Uberlândia
  • Simone Abreu Asmar Universidade Federal de Uberlândia
  • Berildo de Melo Universidade Federal de Uberlândia
  • José Magno Queiroz Luz Universidade Federal de Uberlândia
Keywords: In vitro culture, Culture medium, Explant

Abstract

Savannah is the second biome in biodiversity in Brazil, presenting great vegetation endemism. Baruzeiro (Dipteryx alata Vog.), native from this biome, is an economically important species, with an incipient market due to the lack of commercial plantations. This highlights the need to develop and provide the basis for the domestication of this species. Thus, this study evaluated different concentrations of MS medium, using baruzeiro intact or cut seeds for in vitro establishment. Seeds from baruzeiro ripe fruits were decontaminated and were left intact or partially cut; subsequently, the seeds were inoculated into flasks with different concentrations of MS culture medium. The experimental design was completely randomized as a 5 x 2 factorial, consisting of 5 MS medium concentrations (0, 25, 50, 75 or 100%) and 2 types of seeds (intact or partially cut seeds), with three replications. Each experimental unit consisted of five flasks and 10 plants. After five months of incubation, the contamination of explants, seed germination, the number of fully developed plants and the dry masses of shoot and root of baruzeiros were evaluated. Intact seeds provided better results for all characteristics evaluated. The increased concentration of MS medium resulted in mass gain of plants; however, the use of MS medium 0% provided greater percentage of fully developed plants, the most interesting feature for baruzeiro in vitro establishment.

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Author Biographies

Hernane Fernandes Pinhal, Universidade Federal de Uberlândia
Instituto de Ciências Agrárias, Doutorando em Agronomia
Elequisandra da Costa Araruna, Universidade Federal de Uberlândia
Instituto de Ciências Agrárias
Pedro Augusto Porto Carneiro, Universidade Federal de Uberlândia
Instituto de Ciências Agrárias
Simone Abreu Asmar, Universidade Federal de Uberlândia
Instituto de Ciências Agrárias, Pós-Doutoranda
Berildo de Melo, Universidade Federal de Uberlândia

Instituto de Ciências Agrárias, Professor Titular

 

José Magno Queiroz Luz, Universidade Federal de Uberlândia
Instituto de Ciências Agrárias, Professor Titular
Published
2017-03-30
How to Cite
Pinhal, H. F., Araruna, E. da C., Carneiro, P. A. P., Asmar, S. A., Melo, B. de, & Luz, J. M. Q. (2017). Concentration of ms medium and cutting of seeds on in vitro establishment of baruzeiro (Dipteryx alata Vog.) . Bioscience Journal, 33(2). https://doi.org/10.14393/BJ-v33n2-36327
Section
Agricultural Sciences