Concentration of ms medium and cutting of seeds on in vitro establishment of baruzeiro (Dipteryx alata Vog.)
Savannah is the second biome in biodiversity in Brazil, presenting great vegetation endemism. Baruzeiro (Dipteryx alata Vog.), native from this biome, is an economically important species, with an incipient market due to the lack of commercial plantations. This highlights the need to develop and provide the basis for the domestication of this species. Thus, this study evaluated different concentrations of MS medium, using baruzeiro intact or cut seeds for in vitro establishment. Seeds from baruzeiro ripe fruits were decontaminated and were left intact or partially cut; subsequently, the seeds were inoculated into flasks with different concentrations of MS culture medium. The experimental design was completely randomized as a 5 x 2 factorial, consisting of 5 MS medium concentrations (0, 25, 50, 75 or 100%) and 2 types of seeds (intact or partially cut seeds), with three replications. Each experimental unit consisted of five flasks and 10 plants. After five months of incubation, the contamination of explants, seed germination, the number of fully developed plants and the dry masses of shoot and root of baruzeiros were evaluated. Intact seeds provided better results for all characteristics evaluated. The increased concentration of MS medium resulted in mass gain of plants; however, the use of MS medium 0% provided greater percentage of fully developed plants, the most interesting feature for baruzeiro in vitro establishment.
The copyright for articles published in this journal belong to the authors, with first publication rights granted to the journal. In virtue of their appearance in this journal of open access, the articles are free to use, with proper attribution, in educational and non- commercial applications.
Authors who publish in this journal agree to the following terms:
Authors retain the copyright and waiver the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC-ND 4.0), allowing the sharing of work with authorship recognition and preventing its commercial use.
Authors are authorized to take additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (publish in institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal.