Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

  • Arthur Filipe Sousa Gomes
  • Bruna Silveira Lamanes dos Santos
  • Emanuele Giuliani Franciscon
  • Milla Alves Baffi ICIAG-UFU
Keywords: sugarcane bagasse, wheat brean, corn straw, xylanase, Aspergillus fumigatus

Abstract

This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 oC). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g-1of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts. 

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Published
2016-07-08
How to Cite
Sousa Gomes, A. F., Lamanes dos Santos, B. S., Franciscon, E. G., & Baffi, M. A. (2016). Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes . Bioscience Journal, 32(4). https://doi.org/10.14393/BJ-v32n4a2016-32935
Section
Agricultural Sciences