Antibacterial effect of Herniaria hirsuta, Prunus avium, Rubia tinctorum and Sempervivum tectorum plant extracts on multiple antibiotic resistant Escherichia coli

  • Branislav Radojica Rovčanin Department of human genetics, Faculty of Medicine, University of Belgrade, Višegradska 26, 11000 Belgrade, Serbia
  • Tatjana Department of biochemistry, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia
  • Danijela Steševi Department of biology, Faculty of Natural Science and Mathematics, University of Montenegro, Džordža Vašingtona bb, 81000 Podgorica, Montenegro
  • Dušan Keki Faculty of Medicine, University of Belgrade, Dr Suboti
  • Marko Risti Institute for Molecular Genetics, University of Montpellier 2, Route de Mende 1919, 34293 Montpellier, France
Keywords: Antibiotic resistance, Escherichia coli, Traditional medicine, Plant extracts, Antibacterial compounds

Abstract

The emergence of Escherichia coli isolates with multiple antibiotic resistant phenotypes is considered as a severe health concern. In the present work the antibacterial effect of following plants (Herniaria hirsuta, Prunus avium, Rubia tinctorum and Sempervivum tectorum) was examined. The bacterial model used for estimation of bacterial susceptibility is hospital multiple antibiotic resistant E. coli strain. E. coli ATCC 25922 was used for standard comparison of bacterial susceptibility. Leaves of H. hirsuta, R. tinctorum and S. tectorum as well as petioles of P. avium were collected. Ethanol and aqueous extract of each plant was prepared. Antibacterial activity was examined using the agar well diffusion method. Concentration of total phenols, flavonoids, tannins, antocyanins and saponins was determined in plant extracts. E. coli strain is resistant to four unrelated families of antibiotics. Antibacterial effect is proven for all examined plants. Ethanol extracts of H. hirsuta and P. avium have a more potent antibacterial effect than their aqueous extracts. Aqueous extracts of R. tinctorum and S. tectorum have higher antibacterial potential than theirs ethanol extracts. Examined plant extracts represent good candidates for more extensive research in view of their application in the treatment of multiple antibiotic resistant E.coli strains.  

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Author Biographies

Branislav Radojica Rovčanin, Department of human genetics, Faculty of Medicine, University of Belgrade, Višegradska 26, 11000 Belgrade, Serbia
Teaching assistant, MD, MSc, Specialist of Biology
Tatjana, Department of biochemistry, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia

Professor, PhD, Specialist of Biochemistry

Danijela Steševi, Department of biology, Faculty of Natural Science and Mathematics, University of Montenegro, Džordža Vašingtona bb, 81000 Podgorica, Montenegro
Professor, PhD, Botanist and Plant Physiologist
Dušan Keki, Faculty of Medicine, University of Belgrade, Dr Suboti
MD, MSc, Research assistant in field of Microbiology
Marko Risti, Institute for Molecular Genetics, University of Montpellier 2, Route de Mende 1919, 34293 Montpellier, France
MSc, Research assistant in field of biochemistry and cell biology
Published
2015-11-17
How to Cite
Rovčanin, B. R., Tatjana, Steševi, D., Keki, D., & Risti, M. (2015). Antibacterial effect of Herniaria hirsuta, Prunus avium, Rubia tinctorum and Sempervivum tectorum plant extracts on multiple antibiotic resistant Escherichia coli . Bioscience Journal , 31(6). https://doi.org/10.14393/BJ-v31n6a2015-29091
Section
Health Sciences