New isolate for enhancement production of microbial inulinase

  • Enas Nabil Danial 1-Department of Biochemistry, Faculty of Girls Science, King Abdulaziz University Jeddah, Saudi Arabia. 2Chemistry of Natural and Microbial Products Department, Division of Pharmaceutical industries, National Research Centre, Cairo, Egypt,
  • Nayera A. M. Abdelwahed Chemistry of Natural and Microbial Products Department, Division of Pharmaceutical industries, National Research Centre, Cairo, Egypt,
Keywords: Penicillium funiculosum, biochemical inulinase, inulin, Continuous production

Abstract

The optimization of growth conditions for the production of inulinase by Penicillium funiculosum cells were studied as well as the continuous production of the enzyme using immobilized cells. The highest amount of enzyme (163.5U/mL) was obtained when the producing cells were incubated for 96 hours at 27oC and 200 rpm in a fermentation medium containing both inulin and peptone as sole carbon and nitrogen sources respectively. However, when the cells of the tested microorganism were adsorbed on different carriers, especially linen fibers, their production ability was also successfully maintained, to different extends, for seven successive batches. Moreover, commercially pure inulin is very expensive in only small quantities, this fermentation medium was later substituted by a crude inulin solution obtained from Jerusalem artichoke tubers (Helianthus tuberosus). The crude inulin juice was able to sustain inulinase production during the second batch cultivation of the P. funiculosum, immobilized by their adsorption on linen fibers, in a satisfactory level of about 122U/mL. Moreover, the use of the previously mentioned crude inulin preparation was also compared to the use of either complete or minimal media, composed solely of 1% pure inulin. The method, adopted in this study for inulinase production, is simple, economic, time saving, non-toxic to the microorganism and the loaded linen pads are reusable.

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Author Biographies

Enas Nabil Danial, 1-Department of Biochemistry, Faculty of Girls Science, King Abdulaziz University Jeddah, Saudi Arabia. 2Chemistry of Natural and Microbial Products Department, Division of Pharmaceutical industries, National Research Centre, Cairo, Egypt,

Department of Biochemistry

Nayera A. M. Abdelwahed, Chemistry of Natural and Microbial Products Department, Division of Pharmaceutical industries, National Research Centre, Cairo, Egypt,

Chemistry of Natural and Microbial Products Department,

Published
2015-09-10
How to Cite
Danial, E. N., & Abdelwahed, N. A. M. (2015). New isolate for enhancement production of microbial inulinase . Bioscience Journal, 31(5). https://doi.org/10.14393/BJ-v31n5a2015-28265
Section
Biological Sciences