Molecular characterization and expression pattern of tobacco (Nicotiana tabacum) apoplastic invertase gene
The complete mRNA sequence of one tobacco (Nicotiana tabacum) gene- apoplastic invertase, was amplified using the rapid amplification of cDNA ends methods based on some tobacco ESTs. The full-length tobacco apoplastic invertase gene mRNA was 1,985bp containing an 1,740 bp open reading frame, which encodes a protein of 579 amino acids. Sequence analysis revealed that the apoplastic invertase of tobacco shares high homology with the apoplastic invertase of potato (82%), Lycopersicon esculentum (81%) and Lycopersicon pennellii (78%). Results also showed that tobacco apoplastic invertase gene has a closer genetic relationship with the apoplastic invertase gene of Lycopersicon esculentum. The prediction of transmembrane helices showed that tobacco apoplastic invertase might be a transmembrane protein. The expression profile was studied and the results indicated that tobacco apoplastic invertase gene was differentially expressed in detected tobacco tissues including leaf, stem, root and flower. Our experiment established the foundation for further research on this tobacco gene.
The copyright for articles published in this journal belong to the authors, with first publication rights granted to the journal. In virtue of their appearance in this journal of open access, the articles are free to use, with proper attribution, in educational and non- commercial applications.
Authors who publish in this journal agree to the following terms:
Authors retain the copyright and waiver the journal the right of first publication, with the work simultaneously licensed under the Creative Commons Attribution License (CC BY-NC-ND 4.0), allowing the sharing of work with authorship recognition and preventing its commercial use.
Authors are authorized to take additional contracts separately, for non-exclusive distribution of the version of the work published in this journal (publish in institutional repository or as a book chapter), with acknowledgment of authorship and initial publication in this journal.