Variability of seed-borne Colletotrichum strains in cotton based on ITS1 and ITS2 ribossomal genes analysis

  • Enia Mara de Carvalho Universidade Federal de Lavras
  • Antônia dos Reis Figueira Universidade Federal de Lavras
  • José da Cruz Machado Universidade Federal de Lavras
  • Dejânia Vieira de Araújo Universidade do Estado de Mato Grosso
  • Cibele Ferreira Machado Dow AgroSciences
Keywords: Colletotrichum gossypii, Colletotrichum gossypii var. cephalosporioides, rDNA, phylogeny, Gossypium spp

Abstract

The use of DNA sequences analysis has been an important mean to distinguish and to identify populations of organisms at different levels. By molecular markers several complex organisms have been successful detected in plants for distinct aims. Ribosomal DNA (rDNA) has been used to evaluate genetic variability, microorganism phylogeny and to develop specific primers for detection of plant pathogens in plant tissues. In this study, the objective was to characterize isolates of Colletotrichum gossypii var. cephalosporioides and Colletotrichum gossypii, collected in different regions of Brazil, by analyzing the nucleotide sequence of rDNA regions. ITS1, ITS2, and the intervening 5.8S gene were amplified by PCR and their sequences compared to each other and to those from other species registered in the GenBank. The rDNA of isolates associated with Gossypium spp. showed sequence identities ranging from 96 to 100% in the ITS1 region, 98 to 100% in the 5.8S gene, and 97 to 100% in the ITS2 region. The sequences were submitted to UPGMA analysis, and according to the phylogenetic trees, the C. gossypii var. cephalosporioides and C. gossypii species clustered together along with isolates of Glomerella cingulata from mango and papaya, and thus no distinction could be made between isolates of those organisms.

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Author Biographies

Enia Mara de Carvalho, Universidade Federal de Lavras
Plant Pathology Department, Federal University of Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil
Antônia dos Reis Figueira, Universidade Federal de Lavras
Plant Pathology Department, Federal University of Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil
José da Cruz Machado, Universidade Federal de Lavras
Plant Pathology Department, Federal University of Lavras (UFLA), PO Box 3037, Lavras, MG, 37200-000, Brazil
Dejânia Vieira de Araújo, Universidade do Estado de Mato Grosso
Crop Science Department, Mato Grosso State University (UNEMAT), PO Box 287, Tangará da Serra, MT, 78300-000, Brazil;
Cibele Ferreira Machado, Dow AgroSciences
Biotechnology Laboratory, Dow AgroSciences, PO Box 47, Jardinópolis, SP, 14680-000, Brazil
Published
2015-01-16
Section
Agricultural Sciences